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Perrin S, Wotawa C-A, Luka M, Coulpier F, Masson C, Ménager M and Colnot C. Single nuclei transcriptomics reveal the differentiation trajectories of periosteal skeletal/stem progenitor cells in bone regeneration. 

eLife preprint in review. BioRxiv, 2023 June.


Perrin S and Colnot C. Periosteal Skeletal Stem and Progenitor Cells in Bone Regeneration

Curr Osteoporos Rep. 2022 Oct;20(5):334-343 PMID: 35829950   doi: 10.1007/s11914-022-00737-8

Julien A, Perrin S, Martinez-Sarra, E., Kanagalingam, A., Carvalho C, Luka M., Menager, M. and Colnot, C. Skeletal stem/progenitor cells in periosteum and skeletal muscle share a common molecular response to bone injury

J Bone Miner Res. 2022 June 2. PMID: 35652423   doi: 10.1002/jbmr.4616



Perrin, S, Julien, A, Duchamp de Lageneste O, Abou-Khalil R, Colnot, C. Mouse periosteal cell culture, in vitro differentiation, and in vivo transplantation in tibial fractures. 

Bio Protoc., 11(15): e4107. PMID: 34458401 doi: 10.21769/BioProtoc.4107. eCollection 2021 Aug 5

Julien, A, Kanagalingam, A, Martinez-Sarra, E, Megret, J., Luka M, Menager, M, Relaix, F, Colnot, C. Direct contribution of skeletal muscle mesenchymal progenitors to bone repair.

Nature Communications, 12(1): 2860. PMID: 34001878 doi: 10.1038/s41467-021-22842-5 

Julien, A, Perrin S, Abou-Khalil R, Colnot C. Renal capsule transplantation to assay angiogenesis in skeletal development.

Methods Mol Biol 2230:151-165. PMID:33197014


Julien, A, Perrin S, Duchamp de Lageneste O, Carvalho C, Bensidhoum M, Legeai-Mallet L, Colnot C. FGFR3 in periosteal cells drives cartilage-to-bone transformation in bone repair.

Stem Cell Reports, 2020 Oct 13;15(4):955-967. PMID:32916123

O’Keefe, RJ, Tuan, RS, Lane, NE, Awad, HA, Barry, F, Bunnell, BA, Colnot, C, Drake, MT, Drissi, H, Dyment, NA, Fortier, LA, Guldberg, RE, Kandel, R, Little, DG, Marshall, MF, Mao, JJ, Nakamura, N, Proffen, BL, Rodeo, SA, Rosen, V, Thomopoulos, S, Schwarz, EM, Serra, R. American society for bone and mineral research-orthopaedic research society joint task force report on cell-based therapies-secondary publication.

J Orthop Res, 38(3): 485-502. PMID: 31994782


O’Keefe, RJ, Tuan, RS, Lane, NE, Awad, HA, Barry, F, Bunnell, BA, Colnot, C, Drake, MT, Drissi, H, Dyment, NA, Fortier, LA, Guldberg, RE, Kandel, R, Little, DG, Marshall, MF, Mao, JJ, Nakamura, N, Proffen, BL, Rodeo, SA, Rosen, V, Thomopoulos, S, Schwarz, EM, Serra, R. American society for bone and mineral research-orthopaedic research society joint task force report on cell-based therapies.

 J Bone Miner Res, 35(1): 3-17. PMID: 31545883




Duchamp de Lageneste O, Colnot C. Periostin in bone regeneration.

Adv Exp Med Biol, 1132: 49-61. PMID: 31037624




Duchamp de Lageneste O, Julien A, Abou-Khalil R, Frangi G, Carvalho C, Cagnard N, Cordier C, Conway SJ, Colnot C. Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin.

Nature Communications, 9(1): 773. PMID: 29472541




Stantzou A, Schirwis E, Swist S, Alonso-Martin S, Polydorou I, Zarrouki F, Mouisel E, Beley C, Julien A, Le Grand F, Garcia L, Colnot C, Birchmeier C, Braun T, Schuelke M, Relaix F, Amthor H. BMP signaling regulates satellite cell-dependent postnatal muscle growth.

Development, 144(15): 2737-47. PMID: 28694257




Wang L, Hsiao EC, Lieu S, Scott M, O'Carroll D, Urrutia A, Conklin BR, Colnot C, Nissenson RA. Loss of Gi G-protein-coupled receptor signaling in osteoblasts accelerates bone fracture healing.

J Bone Miner Res, 30(10): 1896-904 PMID: 25917236

Abou-Khalil R, Yang F, Lieu S, Julien A, Perry J, Pereira C, Relaix F, Miclau T, Marcucio R, Colnot C. Role of muscle stem cells during skeletal regeneration.

Stem Cells, 33(5): 1501-11. PMID: 25594525

Abou-Khalil R, Colnot C. Genetically modified models for bone repair.

In Experimental Research Methods (eds. H. Simpson and P. Augat) Thieme Stuttgart-New York.




Abou-Khalil R, Colnot C. Cellular and molecular bases of skeletal regeneration: what can we learn from genetic mouse models?

Bone, 64: 211-21. PMID: 24709685


Abou-Khalil R, Colnot C. Renal capsule transplantations to assay skeletal angiogenesis.

Methods Mol Biol, 1130: 99-110. PMID: 24482168


Abou-Khalil R, Yang F, Mortreux M, Lieu S, Yu YY, Wurmser M, Pereira C, Miclau T, Marcucio R, Colnot  C. Delayed bone regeneration is linked to chronic inflammation in murine muscular dystrophy.

J Bone Miner Res, 29(2): 304-15. PMID: 23857747




Evans S, Docheva D, Colnot C, Richter R, Knothe Tate ML. Solid-supported lipid bilayers to drive stem cell fate and tissue architecture using periosteum derived progenitor cells.

Biomaterials, 34(8): 1878-87. PMID: 23237517


Wang X, Yu YY, Lieu S, Yang F, Lang J, Lu C, Werb Z, Hu D, Miclau T, Marcucio R, Colnot C. MMP9 regulates the cellular response to inflammation after skeletal injury.

Bone, 52(1): 111-19. PMID: 23010105




Colnot C, Zhang X, Knothe Tate ML. Current insights on the regenerative potential of the periosteum: molecular, cellular and endogenous engineering approaches.

J Orthop Res, 30(12): 1869-78. PMID: 22778049


Yu YY, Lieu S, Hu D, Miclau T, Colnot C. Site specific effects of zoledronic acid during tibial and mandibular fracture repair.

PLoS One, 7(2): e31771. PMID: 22359627




Colnot C. Cell sources for bone tissue engineering: insights from basic science.

Tissue Eng Part B Rev, 17(6): 449-57. PMID: 21902612


Lu C, Saless N, Hu D, Wang X, Xing Z, Hou H, Williams B, Swartz HM, Colnot C, Miclau T, Marcucio RS. Mechanical stability affects angiogenesis during early fracture healing.

J Orthop Trauma, 25(8): 494-9. PMID: 21738063


Lieu S, Hansen E, Dedini R, Behonick D, Werb Z, Miclau T, Marcucio R, Colnot C. Impaired remodeling phase of fracture repair in the absence of matrix metalloproteinase-2.

Dis Model Mech, 4(2): 203-11. PMID: 21135956





Pape HC, Marcucio R, Humphrey C, Colnot C, Knobe M, Harvey EJ. Trauma-induced inflammation and fracture healing.

J Orthop Trauma, 24(9): 522-5. PMID: 20736786


Xing Z, Lu C, Hu D, Yu YY, Wang X, Colnot C, Nakamura M, Wu Y, Miclau T, Marcucio RS. Multiple roles of CCR2 during fracture healing.

Dis Model Mech, 3(7-8): 451-8. PMID: 20354109


Yu YY, Lieu S, Lu C, Colnot C. Bone morphogenetic protein 2 stimulates endochondral ossification by regulating periosteal cell fate during bone repair.

Bone, 47(1): 65-73. PMID: 20348041


Lu C, Xing Z, Yu YY, Colnot C, Miclau T, Marcucio RS. Recombinant human bone morphogenetic protein-7 enhances fracture healing in an ischemic environment.

J Orthop Res, 28(5): 687-96. PMID: 19918910


Yu YY, Lieu S, Lu C, Miclau T, Marcucio RS, Colnot C. Immunolocalization of BMPs, BMP antagonists, receptors and effectors during fracture repair.

Bone, 46(3): 841-851. PMID: 19913648


Colnot C, Alliston, T. Tissue interactions in long bone development in topics.

In Bone Biology 6, Bone and Development (eds. F. Bronner, H. Roach and C. Farach-Carson), Springer-Verlag London, UK. 2010: 25-37.





Lu C, Buckley JM, Colnot C, Marcucio R, Miclau T. Basic research in orthopaedic surgery: current trends and future directions.

Indian J Orthop, 43(4): 318-23. PMID: 19838378


Colnot, C. Skeletal cell fate decisions within periosteum and bone marrow during bone regeneration.

J Bone Miner Res, 24: 274-282. PMID: 18847330




Hu D, Colnot C, Marcucio RS. Effect of Bmp signaling on the developing jaw skeleton.

Dev Dyn, 237(12): 3727-37. PMID: 18985754




Behonick DJ, Xing Z, Lieu S, Buckley JM, Lotz JC, Marcucio RS, Werb Z, Miclau T, Colnot C. Role of Matrix metalloproteinase 13 in both endochondral and intramembranous ossification during skeletal regeneration.

PLoS ONE, 2(11):e1150. PMID: 17987127


Miclau T, Bozic K, Tay B, Kim H, Colnot C, Puttlitz C, Gan J, Boyan B, Eames B, Helms J. Bone injury, regeneration, and repair. Orthopaedic basic science.

Am Ac Orthop Surgeons, 18: 341-348.


Colnot C, Romero DM, Huang S, Rahman J, Currey JA, Nanci A, Brunski JB, Helms JA. Molecular analysis of healing at bone-implant interface.

J Dent Res, 86(9): 862-867. PMID: 15930923


Henderson JH, de la Fuente L, Romero D, Colnot CI, Huang S, Carter DR, Helms JA. Rapid growth of cartilage rudiments may generate perichondrial structures by mechanical induction.

Biomech Model Mechanobiol, 6(1-2): 127-37. PMID: 16691413



Colnot C, Huang S, Hlems JA. Analyzing the cellular contribution of bone marrow to fracture healing using bone marrow transplantation in mice.

Biochem Biophys Res Commun, 350(3): 557-61. PMID: 17022937




Ortega N, Behonick DJ, Colnot C, Cooper DN, Werb Z. Galectin-3 is a downstream regulator of MMP-9 function during endochondral bone formation.

Mol Biol Cell, 16: 3028-39. PMID: 15800063


Colnot C, Romero DM, Huang S, Helms JA. Mechanisms of action of demineralized bone matrix in the repair of cortical bone defects.

Clin Orthop Relat Res, 435: 69-78. PMID: 15930923


Colnot C, de la Fuente L, Huang S, Hu D, Lu C, St-Jacques B, Helms JA. Indian Hedgehog synchronizes skeletal angiogenesis and perichondrial maturation with cartilage development.

Development, 132(5): 1057-67. PMID: 15689378


Colnot, C. Cellular and molecular interactions regulating skeletogenesis.

J Cell Biochem, 95: 688-97.  PMID: 15880692




Lu C, Huang S, Miclau T, Helms JA, Colnot C. MEPE is expressed during long bone skeletogenesis and regeneration.

Histochem Cell Biol, 121(6): 493-9. PMID: 15221418


Colnot C, Lu C, Hu D, Helms JA. Distinguishing the contributions of the perichondrium, cartilage, and vascular endothelium to skeletal development.

Dev Biol, 269: 55-69. PMID: 15081357




Colnot C, Thompson Z, Miclau T, Werb Z, Helms JA. Altered fracture repair in the absence of MMP9.

Development, 130: 4123-4133. PMID: 12874132


Miclau T, Colnot C, Helms JA. Molecular regulation of bone regeneration.

In Molecular Biology in Orthopaedics, (ed. R. N. Rosier and C. H. Evans), pp. 251-262. Rosemont, IL: American Academy of Orthopaedic Surgeons.




Colnot CI, Helms JA. A molecular analysis of matrix remodeling and angiogenesis during long bone development.

Mech Dev, 100: 245-250. PMID: 11165481


Colnot C, Sidhu SS, Balmain N, Poirier F. Uncoupling of chondrocyte death and vascular invasion in galectin-3 null mutant fetal bones.

Dev Biol, 229: 203-214 . PMID: 11133164

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